Device

Part:BBa_K2428001

Designed by: Patricia Zulueta   Group: iGEM17_UChicago   (2017-10-26)


Yeast-Bacteria shuttle vector

This part contains one of our other parts (BBa_K2428000, which is psB1C3mut), SCARG4 (a gene that produces arginine) and PARS2 (an autonomous replicating element). We PCRd the region containing these two genes. Using Gibson assembly, we added this part into psB1C3mut, resulting in psD000. The presence of SCARG4 and PARS2 in our construct means that psD000 is a shuttle vector (it can be transformed and expressed into both E. coli and yeast). Adding this part into any iGEM plasmid backbone will make it into a shuttle vector for Pichia pastoris and E. coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1602
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1767
    Illegal XhoI site found at 3568
    Illegal XhoI site found at 4460
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 820
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1004
    Illegal SapI.rc site found at 448


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Categories
Parameters
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